UniProt:Q6AU80 ISA2

chain
  • transit peptide:1-34
  • chain:35-800
checksum 97F4019B0783508A
comment
  • FUNCTION Starch-debranching enzyme involved in amylopectin biosynthesis in endosperm. Functions by removing excess branches or improper branches that interfere with the formation of double helices of the cluster chains of amylopectin and crystallization of starch (PubMed:16953433, PubMed:21436381). Works together with ISA1 as heterooligomer. The heterooligomer ISA1 and ISA2 possesses higher affinity than the ISA1 homooligomer for various branched polyglucans in vitro, but no marked differences exist in chain preferences for debranching of amylopectin and phytoglycogen between these forms (PubMed:16953433, PubMed:21436381).CATALYTIC ACTIVITY Hydrolysis of (1->6)-alpha-D-glucosidic branch linkages in glycogen, amylopectin and their beta-limit dextrins.SUBUNIT Forms a hetero-hexamer composed of five ISA1 and one ISA2.SUBCELLULAR LOCATION Highly expressed in developing endosperm and leaves.SIMILARITY Belongs to the glycosyl hydrolase 13 family.SEQUENCE CAUTION Truncated N-terminus.SEQUENCE CAUTION Truncated N-terminus.SEQUENCE CAUTION Truncated N-terminus.
created [InstanceEdit:9918269] Naithani, Sushma, 2024-08-14
databaseName UniProt
dbId 9918262
description
  • recommendedName: fullName evidence="7"Isoamylase 2, chloroplastic shortName evidence="6"OsISA2 ecNumber evidence="4"3.2.1.68
displayName UniProt:Q6AU80 ISA2
geneName
  • ISA2
  • Os05g0393700
  • LOC_Os05g32710
  • OsJ_17304
  • OSJNBa0014C03.3
identifier Q6AU80
isSequenceChanged false
keyword
  • Carbohydrate metabolism
  • Chloroplast
  • Direct protein sequencing
  • Glycosidase
  • Hydrolase
  • Plastid
  • Reference proteome
  • Starch biosynthesis
  • Transit peptide
modified [InstanceEdit:9926675] Weiser, Joel, 2024-11-03
name
  • ISA2
referenceDatabase [ReferenceDatabase:2] UniProt
schemaClass ReferenceGeneProduct
secondaryIdentifier
  • ISOA2_ORYSJ
  • A0A0P0WM70
  • B9FHZ3
  • Q0DIF3
sequenceLength 800
species [Species:1250427] Oryza sativa subsp. japonica
url http://purl.uniprot.org/uniprot/Q6AU80