UniProt:Q84ZL5 Os08g0154600

comment
  • FUNCTION: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at the specific target site 5'-[CT]CCTTp site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. {ECO:0000256|RuleBase:RU365101}.
  • CATALYTIC ACTIVITY: Reaction=ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.; EC=5.6.2.1; Evidence={ECO:0000256|RuleBase:RU365101};
created [InstanceEdit:9675413] Gupta, Parul, 2020-02-04
databaseName UniProt
dbId 9675317
displayName UniProt:Q84ZL5 Os08g0154600
geneName
  • Os08g0154600
  • OJ1066_B03.125
  • OsJ_26075
  • OSNPB_080154600
identifier Q84ZL5
keyword
  • Coiled coil
  • DNA-binding
  • Isomerase
  • Reference proteome
  • Topoisomerase
modified [InstanceEdit:9675682] Gupta, Parul, 2020-02-04
referenceDatabase [ReferenceDatabase:2] UniProt
schemaClass ReferenceGeneProduct
secondaryIdentifier
  • Q84ZL5_ORYSJ
species [Species:1250427] Oryza sativa subsp. japonica
url http://purl.uniprot.org/uniprot/Q84ZL5