UniProt:Q0DBF4 Os06g0569500

chain
  • chain:1-510
checksum 7FA05F6A42E664E8
comment
  • FUNCTION Catalyzes the hydroxylation of ent-sandaracopimaradiene at the C3alpha position to produce ent-3beta-hydroxy-sandaracopimaradiene, an intermediates for the biosynthesis of oryzalexin D and oryzalexin E phytoalexins (PubMed:22247270, PubMed:23795884). Catalyzes the hydroxylation of ent-cassadiene at the C3alpha position to produce 3alpha-hydroxy-ent-cassadiene, which may be an intermediate for the biosynthesis of phytocassane phytoalexins (PubMed:22247270). Catalyzes the hydroxylation of syn-pimaradiene (9-beta-pimara-7,15-diene) at the C3beta position to produce 3-beta-syn-pimaradiene (PubMed:25758958). Can hydroxylate ent-kaurene in vitro, but the product is not ent-kauren-19-ol as expected for ent-kaurene oxidase activity (PubMed:22247270).CATALYTIC ACTIVITY ent-sandaracopimara-8(14),15-diene + reduced [NADPH--hemoprotein reductase] + O2 = ent-sandaracopimaradien-3beta-ol + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)CATALYTIC ACTIVITY 9beta-pimara-7,15-diene + reduced [NADPH--hemoprotein reductase] + O2 = 9beta-pimara-7,15-diene-3beta-ol + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)COFACTOR kcat is 1.4 sec(-1) with syn-pimaradiene as substrate.SUBUNIT Interacts with the rice dwarf virus (RDV) P2 protein.SUBCELLULAR LOCATION Expressed in leaf blades and sheaths, stems and panicles.INDUCTION By N-acetylchitooligosaccharide elicitor (PubMed:15316288) and methyl jasmonate (PubMed:22247270).SIMILARITY Belongs to the cytochrome P450 family.
created [InstanceEdit:1164949] Wu, Guanming, 2010-12-11
crossReference
databaseName UniProt
dbId 1160922
description
  • recommendedName: fullName evidence="12"Ent-sandaracopimaradiene 3-hydroxylase ecNumber evidence="6 7"1.14.14.70 alternativeName: fullName evidence="12"Cytochrome P450 701A8 alternativeName: fullName evidence="9"Ent-kaurene oxidase 4 shortName evidence="9"OsKO4 alternativeName: fullName evidence="10"Ent-kaurene oxidase-like 4 shortName evidence="10"OsKOL4 alternativeName: fullName evidence="11"OsKOS1 alternativeName: fullName evidence="13"Syn-pimaradiene 3-monooxygenase ecNumber evidence="8"1.14.14.68
displayName UniProt:Q0DBF4 Os06g0569500
geneName
  • Os06g0569500
  • CYP701A8
  • KO1
  • LOC_Os06g37300
  • OSJNBa0062E01.27
identifier Q0DBF4
isSequenceChanged false
keyword
  • Heme
  • Host-virus interaction
  • Iron
  • Membrane
  • Metal-binding
  • Monooxygenase
  • NADP
  • Oxidoreductase
  • Reference proteome
  • Transmembrane
  • Transmembrane helix
modified [InstanceEdit:9939033] Weiser, Joel, 2025-02-21
name
  • CYP701A8
referenceDatabase [ReferenceDatabase:2] UniProt
schemaClass ReferenceGeneProduct
secondaryIdentifier
  • C7018_ORYSJ
  • A0A0N7KMA9
  • Q5Z5S0
  • Q6GZ42
sequenceLength 510
species [Species:1250427] Oryza sativa subsp. japonica
url http://purl.uniprot.org/uniprot/Q0DBF4