UniProt:Q5VQG4 RFS

chain
  • initiator methionine:
  • chain:2-783
checksum 59BB443A5965DC1D
comment
  • FUNCTION Transglycosidase operating by a ping-pong reaction mechanism. Involved in the synthesis of raffinose, a major soluble carbohydrate in seeds, roots and tubers. Specific for galactinol and p-nitrophenyl-alpha-D-galactoside as galactosyl donors. Able to utilize sucrose, lactose, 4-beta-galactobiose, N-acetyl-D-lactosamine, trehalose and lacto-N-biose as acceptors. May also act as a glycoside hydrolase.CATALYTIC ACTIVITY alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose = raffinose + myo-inositolACTIVITY REGULATION Inhibited by Ag(2)+, Hg(2+), Zn(2+), p-chloromercuribenzoate (pCMB) and 1-deoxygalactonojirimycin.BIOPHYSICOCHEMICAL PROPERTIES Optimum pH is 7.0.SIMILARITY Belongs to the glycosyl hydrolases 36 family.
created [InstanceEdit:1164949] Wu, Guanming, 2010-12-11
crossReference
databaseName UniProt
dbId 1160730
description
  • recommendedName: Galactinol--sucrose galactosyltransferase ecNumber: 2.4.1.82 alternativeName: Raffinose synthase
displayName UniProt:Q5VQG4 RFS
geneName
  • RFS
  • Os01g0170000
  • LOC_Os01g07530
  • OJ1276_B06.38
  • OsJ_00538
  • P0583G08.2
identifier Q5VQG4
isSequenceChanged false
keyword
  • Carbohydrate metabolism
  • Direct protein sequencing
  • Glycosyltransferase
  • Reference proteome
  • Transferase
modified [InstanceEdit:9939033] Weiser, Joel, 2025-02-21
name
  • RFS
referenceDatabase [ReferenceDatabase:2] UniProt
schemaClass ReferenceGeneProduct
secondaryIdentifier
  • RFS_ORYSJ
  • Q0JQC7
sequenceLength 783
species [Species:1250427] Oryza sativa subsp. japonica
url http://purl.uniprot.org/uniprot/Q5VQG4