UniProt:Q94GF1 ASA1

chain
  • transit peptide:1-34
  • chain:35-577
checksum 2B8E72EB0846EA95
comment
  • FUNCTION Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS to produce anthranilate.CATALYTIC ACTIVITY chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate + H(+)ACTIVITY REGULATION Feedback inhibition by tryptophan.BIOPHYSICOCHEMICAL PROPERTIES kcat is 34.7 sec(-1) with chorismate as substrate (for recombinant ASA1 and ASB1 proteins synthesized with the wheat germ cell-free system).PATHWAY Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.SUBUNIT Heterotetramer consisting of two non-identical subunits: a beta subunit and a large alpha subunit.SUBCELLULAR LOCATION No visible phenotype under normal growth conditions, but mutant plants are insensitive to feedback inhibition by tryptophan.SIMILARITY Belongs to the anthranilate synthase component I family.
created [InstanceEdit:1164949] Wu, Guanming, 2010-12-11
crossReference
databaseName UniProt
dbId 1160226
description
  • recommendedName: fullName evidence="5"Anthranilate synthase alpha subunit 1, chloroplastic shortName evidence="5"OsASA1 ecNumber evidence="4"4.1.3.27
displayName UniProt:Q94GF1 ASA1
geneName
  • ASA1
  • OASA1
  • Os03g0826500
  • LOC_Os03g61120
  • OJ1111_B11.9
  • OsJ_13197
  • OSJNBa0010E04.11
identifier Q94GF1
isSequenceChanged false
keyword
  • Amino-acid biosynthesis
  • Aromatic amino acid biosynthesis
  • Chloroplast
  • Lyase
  • Plastid
  • Reference proteome
  • Transit peptide
  • Tryptophan biosynthesis
modified [InstanceEdit:9939033] Weiser, Joel, 2025-02-21
name
  • ASA1
referenceDatabase [ReferenceDatabase:2] UniProt
schemaClass ReferenceGeneProduct
secondaryIdentifier
  • ASA1_ORYSJ
  • A0A0P0W4Y1
  • Q10B97
  • Q9XJ30
sequenceLength 577
species [Species:1250427] Oryza sativa subsp. japonica
url http://purl.uniprot.org/uniprot/Q94GF1