UniProt:Q6ZD89 COMT

chain
  • propeptide:1-2
  • chain:3-368
checksum 2491B4FA7447558E
comment
  • FUNCTION Methylates OH residues of flavonoid compounds. Can methylate eriodictyol, luteolin, quercetin and taxifolin (PubMed:16412485). Methylates caffeate to produce ferrulate (PubMed:21210840, Ref.16). Catalyzes the methylation of monolignols, the lignin precursors. Functions cooperatively with CAD2 in the culm internodes for the biosynthesis of monolignols. May be involved in lignin biosynthesis in leaves and roots (PubMed:21912859). Involved in syringyl lignin biosynthesis. Can function as 5-hydroxyconiferaldehyde O-methyltransferase in the biosynthetic pathway to syringyl lignin (Ref.16). Involved in melatonin biosynthesis. Can function as acetylserotonin O-methyltransferase. Catalyzes the transfer of a methyl group onto N-acetylserotonin, producing melatonin (N-acetyl-5-methoxytryptamine) (PubMed:26276868).CATALYTIC ACTIVITY a 3'-hydroxyflavone + S-adenosyl-L-methionine = a 3'-methoxyflavone + S-adenosyl-L-homocysteine + H(+)CATALYTIC ACTIVITY N-acetylserotonin + S-adenosyl-L-methionine = melatonin + S-adenosyl-L-homocysteine + H(+)CATALYTIC ACTIVITY (E)-caffeate + S-adenosyl-L-methionine = (E)-ferulate + S-adenosyl-L-homocysteine + H(+)ACTIVITY REGULATION Acetylserotonin O-methyltransferase activity is inhibited by quercetin and caffeate.BIOPHYSICOCHEMICAL PROPERTIES Measured at pH 7.5 and 37 degrees Celsius for all experiments.PATHWAY Flavonoid metabolism; quercetin degradation.SUBUNIT Monomer.SUBCELLULAR LOCATION Expressed in roots and stems, and at lower levels in leaves.INDUCTION By probenazole (at protein level) (PubMed:17950386). Induced by UV-C (PubMed:24035516).SIMILARITY Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O-methyltransferase family. COMT subfamily.CAUTION Was initially thought to constitute the naringenin 7-O-methyltransferase (NOMT), a methyltransferase involved in the biosynthesis of the sakuranetin, an inducible defense mechanism of O.sativa against pathogen attack (PubMed:10814825). However, it was later shown that it is not the case (PubMed:22493492).SEQUENCE CAUTION Truncated N-terminus.
created [InstanceEdit:1164949] Wu, Guanming, 2010-12-11
crossReference
databaseName UniProt
dbId 1159587
description
  • recommendedName: fullName evidence="16"Flavone 3'-O-methyltransferase 1 shortName evidence="16"OsOMT1 ecNumber evidence="5"2.1.1.42 alternativeName: fullName evidence="16"Acetylserotonin O-methyltransferase COMT ecNumber evidence="11"2.1.1.4 alternativeName: fullName evidence="16"Caffeate O-methyltransferase 1 shortName evidence="15"OsCOMT1 ecNumber evidence="12"2.1.1.68 alternativeName: fullName evidence="14"OsCOMT alternativeName: fullName evidence="16"Quercetin 3'-O-methyltransferase 1
displayName UniProt:Q6ZD89 COMT
geneName
  • COMT
  • ROMT-9
  • Os08g0157500
  • LOC_Os08g06100
  • OsJ_26105
  • P0438H08.29
identifier Q6ZD89
isSequenceChanged false
keyword
  • Cytoplasm
  • Direct protein sequencing
  • Melatonin biosynthesis
  • Methyltransferase
  • Reference proteome
  • S-adenosyl-L-methionine
  • Transferase
modified [InstanceEdit:9939033] Weiser, Joel, 2025-02-21
name
  • COMT
referenceDatabase [ReferenceDatabase:2] UniProt
schemaClass ReferenceGeneProduct
secondaryIdentifier
  • OMT1_ORYSJ
  • A0A0P0XBV5
  • A3BPT2
  • Q19BJ6
  • Q75W57
sequenceLength 368
species [Species:1250427] Oryza sativa subsp. japonica
url http://purl.uniprot.org/uniprot/Q6ZD89