UniProt:Q2QWK9 Os12g0192500

chain
  • transit peptide:1-47
  • chain:48-548
checksum 9B5204B75835A72E
comment
  • FUNCTION Essential for thiamine biosynthesis. Bifunctional enzyme that catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP and condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP).CATALYTIC ACTIVITY 2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H(+) = thiamine phosphate + CO2 + diphosphateCATALYTIC ACTIVITY 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H(+) = thiamine phosphate + CO2 + diphosphateCATALYTIC ACTIVITY 4-methyl-5-(2-phosphooxyethyl)-thiazole + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + H(+) = thiamine phosphate + diphosphateCATALYTIC ACTIVITY 4-amino-5-hydroxymethyl-2-methylpyrimidine + ATP = 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + ADP + H(+)COFACTOR Binds 1 Mg(2+) ion per subunit.PATHWAY Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine phosphate from 4-amino-2-methyl-5-diphosphomethylpyrimidine and 4-methyl-5-(2-phosphoethyl)-thiazole: step 1/1.PATHWAY Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-amino-2-methyl-5-diphosphomethylpyrimidine from 5-amino-1-(5-phospho-D-ribosyl)imidazole: step 2/3.SUBCELLULAR LOCATION Belongs to the thiamine-phosphate synthase family.
created [InstanceEdit:1164949] Wu, Guanming, 2010-12-11
crossReference
databaseName UniProt
dbId 1159544
description
  • recommendedName: Probable thiamine biosynthetic bifunctional enzyme, chloroplastic domain recommendedName: Thiamine-phosphate synthase shortName: TP synthase shortName: TPS ecNumber evidence="3"2.5.1.3 alternativeName: Thiamine-phosphate pyrophosphorylase shortName: TMP pyrophosphorylase shortName: TMP-PPase /domain domain recommendedName: Hydroxymethylpyrimidine kinase shortName: HMP kinase ecNumber evidence="2"2.7.1.49 /domain
displayName UniProt:Q2QWK9 Os12g0192500
geneName
  • Os12g0192500
  • LOC_Os12g09000
  • OsJ_35490
identifier Q2QWK9
isSequenceChanged false
keyword
  • ATP-binding
  • Chloroplast
  • Kinase
  • Magnesium
  • Metal-binding
  • Multifunctional enzyme
  • Nucleotide-binding
  • Plastid
  • Reference proteome
  • Thiamine biosynthesis
  • Transferase
  • Transit peptide
modified [InstanceEdit:9939033] Weiser, Joel, 2025-02-21
name
  • Os12g0192500
referenceDatabase [ReferenceDatabase:2] UniProt
schemaClass ReferenceGeneProduct
secondaryIdentifier
  • TPS1_ORYSJ
  • A0A0P0Y7Y2
  • Q0IPK3
sequenceLength 548
species [Species:1250427] Oryza sativa subsp. japonica
url http://purl.uniprot.org/uniprot/Q2QWK9