UniProt:Q8LM92 LOC_Os10g16974

chain
  • chain:1-535
checksum 9AB17D2B9A86B6F1
comment
  • FUNCTION Catalyzes the 3'-hydroxylation of the flavonoid B-ring to the 3',4'-hydroxylated state. Catalyzes in vitro 3'-hydroxylation of different flavonoids. Catalyzes the conversion of apigenin to luteolin, naringenin to eriodictyol, and kaempferol to quercetin. Possesses specific 5'-hydroxylase activity toward chrysoeriol (a 3'-methoxylated flavone) and is indispensable for tricin formation. Converts chrysoeriol to selgin, a precursor of tricin, suggesting that chrysoeriol, instead of tricetin, is an intermediate in tricin biosynthesis.CATALYTIC ACTIVITY a 3'-unsubstituted flavone + reduced [NADPH--hemoprotein reductase] + O2 = a 3'-hydroxyflavone + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)COFACTOR Secondary metabolite biosynthesis; flavonoid biosynthesis.SUBCELLULAR LOCATION Belongs to the cytochrome P450 family.
created [InstanceEdit:1164949] Wu, Guanming, 2010-12-11
crossReference
databaseName UniProt
dbId 1159259
description
  • recommendedName: fullName evidence="5"Flavonoid 3'-monooxygenase CYP75B4 ecNumber evidence="3"1.14.14.82 alternativeName: fullName evidence="4"Chrysoeriol 5'-hydroxylase alternativeName: fullName evidence="4"Cytochrome P450 75B4 alternativeName: fullName evidence="4"Flavonoid 3'-hydroxylase CYP75B4
displayName UniProt:Q8LM92 LOC_Os10g16974
geneName
  • LOC_Os10g16974
  • OSJNBb0079E01.10
  • Os10g0317900
  • CYP75B4
identifier Q8LM92
isSequenceChanged false
keyword
  • Flavonoid biosynthesis
  • Heme
  • Iron
  • Membrane
  • Metal-binding
  • Monooxygenase
  • NADP
  • Oxidoreductase
  • Reference proteome
  • Transmembrane
  • Transmembrane helix
modified [InstanceEdit:9939033] Weiser, Joel, 2025-02-21
name
  • CYP75B4
referenceDatabase [ReferenceDatabase:2] UniProt
schemaClass ReferenceGeneProduct
secondaryIdentifier
  • C75B4_ORYSJ
sequenceLength 535
species [Species:1250427] Oryza sativa subsp. japonica
url http://purl.uniprot.org/uniprot/Q8LM92