UniProt:Q5CCK3 CYP90B2

chain
  • chain:1-506
checksum 68E733DA80556B9F
comment
  • FUNCTION Catalyzes the C22-alpha-hydroxylation step in brassinosteroid biosynthesis, which is the rate-limiting step in this biosynthetic pathway (PubMed:16369540). Catalyzes the conversion of campesterol (CR) to (22S)-22-hydroxycampesterol (22-OHCR, 22-hydroxyCR) and of campestanol (CN) to 6-deoxycathasterone (6-deoxoCT) (PubMed:16369540). Required for auxin responses involved in the regulation of epidermal cells length of the lamina joint (PubMed:19605414).CATALYTIC ACTIVITY a C28-steroid + reduced [NADPH--hemoprotein reductase] + O2 = a (22S)-22-hydroxy C28-steroid + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)CATALYTIC ACTIVITY campesterol + reduced [NADPH--hemoprotein reductase] + O2 = (22S)-22-hydroxycampesterol + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)CATALYTIC ACTIVITY campestanol + reduced [NADPH--hemoprotein reductase] + O2 = 6-deoxycathasterone + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)COFACTOR Plant hormone biosynthesis; brassinosteroid biosynthesis.SUBCELLULAR LOCATION Highly expressed in roots and leaf blades. Expressed in shoot apex, stems, leaf sheaths, inflorescences and flowers.INDUCTION Down-regulated by brassinolide (BL).DISRUPTION PHENOTYPE Slight dwarf phenotype and erect leaves (PubMed:16369540). Impaired response to auxin leading to reduced epidermal cells length of the lamina joint (PubMed:19605414).BIOTECHNOLOGY Null mutant plants for CYP90B1/DWARF4 produce increased biomass and grain yield at high density planting.SIMILARITY Belongs to the cytochrome P450 family.SEQUENCE CAUTION Truncated N-terminus.
created [InstanceEdit:1164949] Wu, Guanming, 2010-12-11
crossReference
databaseName UniProt
dbId 1157127
description
  • recommendedName: fullName evidence="7"Steroid (22S)-hydroxylase ecNumber evidence="3"1.14.14.178 alternativeName: fullName evidence="5"(22S)-22-hydroxycampesterol synthase alternativeName: fullName evidence="5"6-deoxycathasterone synthase alternativeName: fullName evidence="7"Cytochrome P450 90B2 shortName evidence="7"OsCYP90B2 alternativeName: fullName evidence="5"Protein DWARF4 homolog shortName evidence="5 6"OsDWARF4 alternativeName: fullName evidence="7"Steroid 22-alpha-hydroxylase
displayName UniProt:Q5CCK3 CYP90B2
geneName
  • CYP90B2
  • CYP90B1
  • DWARF4
  • Os03g0227700
  • LOC_Os03g12660
  • OJ1626B05.9
identifier Q5CCK3
isSequenceChanged false
keyword
  • Brassinosteroid biosynthesis
  • Heme
  • Iron
  • Lipid biosynthesis
  • Lipid metabolism
  • Membrane
  • Metal-binding
  • Monooxygenase
  • Oxidoreductase
  • Reference proteome
  • Steroid biosynthesis
  • Transmembrane
  • Transmembrane helix
modified [InstanceEdit:9939033] Weiser, Joel, 2025-02-21
name
  • CYP90B2
referenceDatabase [ReferenceDatabase:2] UniProt
schemaClass ReferenceGeneProduct
secondaryIdentifier
  • C90B2_ORYSJ
  • Q8H848
sequenceLength 506
species [Species:1250427] Oryza sativa subsp. japonica
url http://purl.uniprot.org/uniprot/Q5CCK3