UniProt:Q6ZKV8 BIO3-BIO1

chain
  • transit peptide:1-
  • chain:-821
checksum 40663C38B8859EEE
comment
  • FUNCTION Bifunctional enzyme that catalyzes two different reactions involved in the biotin biosynthesis.FUNCTION Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring.FUNCTION Catalyzes the transfer of the alpha-amino group from S-adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form 7,8-diaminopelargonic acid (DAPA). It is the only aminotransferase known to utilize SAM as an amino donor (By similarity).CATALYTIC ACTIVITY (7R,8S)-7,8-diammoniononanoate + CO2 + ATP = (4R,5S)-dethiobiotin + ADP + phosphate + 3 H(+)CATALYTIC ACTIVITY (8S)-8-amino-7-oxononanoate + S-adenosyl-L-methionine = S-adenosyl-4-methylsulfanyl-2-oxobutanoate + (7R,8S)-7,8-diammoniononanoateCOFACTOR Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2.PATHWAY Cofactor biosynthesis; biotin biosynthesis; 7,8-diaminononanoate from 8-amino-7-oxononanoate (SAM route): step 1/1.SUBCELLULAR LOCATION In the N-terminal section; belongs to the dethiobiotin synthetase family.SIMILARITY In the C-terminal section; belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. BioA subfamily.
created [InstanceEdit:1164949] Wu, Guanming, 2010-12-11
crossReference
databaseName UniProt
dbId 1156199
description
  • recommendedName: Bifunctional dethiobiotin synthetase/7,8-diamino-pelargonic acid aminotransferase, mitochondrial alternativeName: Bifunctional BIO3-BIO1 protein domain recommendedName: Dethiobiotin synthetase ecNumber: 6.3.3.3 alternativeName: DTB synthetase shortName: DTBS alternativeName: Protein BIOTIN AUXOTROPH 3 /domain domain recommendedName: 7,8-diamino-pelargonic acid aminotransferase shortName: DAPA AT shortName: DAPA aminotransferase alternativeName: 7,8-diaminononanoate synthase shortName: DANS alternativeName: Adenosylmethionine-8-amino-7-oxononanoate aminotransferase ecNumber: 2.6.1.62 alternativeName: Diaminopelargonic acid synthase alternativeName: Protein BIOTIN AUXOTROPH 1 /domain
displayName UniProt:Q6ZKV8 BIO3-BIO1
geneName
  • BIO3-BIO1
  • BIO1
  • BIO3
  • Os08g0245400
  • LOC_Os08g14770
  • OJ1033_B09.17
  • OsJ_26590
identifier Q6ZKV8
isSequenceChanged false
keyword
  • Aminotransferase
  • ATP-binding
  • Biotin biosynthesis
  • Ligase
  • Magnesium
  • Metal-binding
  • Mitochondrion
  • Multifunctional enzyme
  • Nucleotide-binding
  • Pyridoxal phosphate
  • Reference proteome
  • S-adenosyl-L-methionine
  • Transferase
  • Transit peptide
modified [InstanceEdit:9939033] Weiser, Joel, 2025-02-21
name
  • BIO3-BIO1
referenceDatabase [ReferenceDatabase:2] UniProt
schemaClass ReferenceGeneProduct
secondaryIdentifier
  • BIODA_ORYSJ
  • A0A0P0XDC2
sequenceLength 821
species [Species:1250427] Oryza sativa subsp. japonica
url http://purl.uniprot.org/uniprot/Q6ZKV8