UniProt:P14656 GLN1-1

chain
  • chain:1-356
checksum 7D21F7C31B7CB4DD
comment
  • FUNCTION High-affinity glutamine synthetase involved in ammonium assimilation (PubMed:15918879, PubMed:21255162). Seems to be a major component of the cytosolic glutamine synthetic pathway in leaf blades (PubMed:15918879, PubMed:21255162). Plays an important role in maintaining carbon and nitrogen metabolic balance during ammonium assimilation in shoots and roots, thus controlling plant growth and development (PubMed:21255162, PubMed:24743556). Plays an important role in maintaining broad range of metabolites and transcripts involved in the maintenance of plant metabolic homeostasis and development of plastid in roots (PubMed:32024696).CATALYTIC ACTIVITY L-glutamate + NH4(+) + ATP = L-glutamine + ADP + phosphate + H(+)BIOPHYSICOCHEMICAL PROPERTIES Measured at pH 7.8 and 30 degrees Celsius for all experiments.SUBUNIT Homooctamer.SUBCELLULAR LOCATION Highly expressed in leaf blades, at intermediate levels in spikelets (rice flower) and at lower levels in roots.DEVELOPMENTAL STAGE Expressed in metaxylem and phloem of parenchyma cells and in sclerenchyma cells of developing leaf blades.INDUCTION Induced in roots under nitrogen-limited condition (PubMed:15574840, PubMed:23509111). Down-regulated in roots under ammonium supply (PubMed:17350935). Down-regulated by treatment with cadmium (PubMed:23743654).DISRUPTION PHENOTYPE Plants show marked reduction in leaf blades elongation, plant height, panicle size and grain filling.MISCELLANEOUS Plants overexpressing GLN1-1 exhibit a poor plant growth phenotype and yield, and decreased carbon/nitrogen ratio in the stem caused by the accumulation of nitrogen in the stem.SIMILARITY Belongs to the glutamine synthetase family.
created [InstanceEdit:1164949] Wu, Guanming, 2010-12-11
crossReference
databaseName UniProt
dbId 1155941
description
  • recommendedName: fullName evidence="16"Glutamine synthetase cytosolic isozyme 1-1 ecNumber evidence="5"6.3.1.2 alternativeName: fullName evidence="16"Glutamate--ammonia ligase GLN1;1 shortName evidence="13"OsGLN1;1 alternativeName: fullName evidence="16"Glutamine synthetase shoot isozyme alternativeName: fullName evidence="15"OsGS1;1
displayName UniProt:P14656 GLN1-1
geneName
  • GLN1-1
  • RGS28
  • Os02g0735200
  • LOC_Os02g50240
  • OsJ_08295
  • P0487D09.8
identifier P14656
isSequenceChanged false
keyword
  • ATP-binding
  • Cytoplasm
  • Direct protein sequencing
  • Ligase
  • Nucleotide-binding
  • Reference proteome
modified [InstanceEdit:9939033] Weiser, Joel, 2025-02-21
name
  • GLN1-1
referenceDatabase [ReferenceDatabase:2] UniProt
schemaClass ReferenceGeneProduct
secondaryIdentifier
  • GLN11_ORYSJ
  • Q0DXS9
  • Q6Z753
sequenceLength 356
species [Species:1250427] Oryza sativa subsp. japonica
url http://purl.uniprot.org/uniprot/P14656