The gene Os01g0866100 encodes ACT7 protein and plays a significant role in the plant?s response to anaerobic conditions, such as those encountered during flooding. This gene negatively regulates coleoptile length, which is crucial for enhancing tolerance to anaerobic germination ((Yuan et al., 2024). Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Essential component of cell cytoskeleton; plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth.